Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
The...
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RETRACTED: Bakshi et al. Crocin Inhibits Angiogenesis and Metastasis in Colon Cancer via TNF-α/NF-kB/VEGF Pathways. <i>Cells</i> 2022, <i>11</i>, 1502.

Cells·2026
Same author

Sequence-Encoded Frustration Directs the Formation of Abridged G-Quadruplex Architectures.

Angewandte Chemie (International ed. in English)·2026
Same author

Loop topology enables programmable apoptosis in G-quadruplex aptamers.

Bioorganic chemistry·2025
Same author

Correction: Bakshi et al. Dietary Crocin is Protective in Pancreatic Cancer while Reducing Radiation-Induced Hepatic Oxidative Damage. <i>Nutrients</i> 2020, <i>12</i>, 1901.

Nutrients·2025
Same author

RETRACTED: Crocin Inhibits Angiogenesis and Metastasis in Colon Cancer via TNF-α/NF-kB/VEGF Pathways.

Cells·2022
Same author

Correction: Aljabali, A.A.A.; et al. Albumin Nano-Encapsulation of Piceatannol Enhances Its Anticancer Potential in Colon Cancer via down Regulation of Nuclear p65 and HIF-1α. <i>Cancers</i> 2020, <i>12</i>, 113.

Cancers·2020

Related Experiment Video

Updated: Jul 10, 2026

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

NMR methods for studying quadruplex nucleic acids.

Mateus Webba da Silva1

  • 1School of Biomedical Sciences, University of Ulster, Cromore Road, Coleraine BT52 1SA, UK. mm.webba-da-silva@ulster.ac.uk

Methods (San Diego, Calif.)
|October 31, 2007
PubMed
Summary

Solution NMR spectroscopy is key for studying nucleic acid quadruplexes. This overview covers methods for analyzing their structure, dynamics, thermodynamics, and kinetics, including associated cations.

Area of Science:

  • Biophysical Chemistry
  • Structural Biology
  • Molecular Biophysics

Background:

  • Solution Nuclear Magnetic Resonance (NMR) spectroscopy is a cornerstone technique in molecular biology.
  • Nucleic acid quadruplexes are G-rich structures with significant biological relevance.
  • Understanding quadruplexes is crucial for drug discovery and understanding genetic regulation.

Purpose of the Study:

  • To provide a comprehensive overview of NMR spectroscopy methods for nucleic acid quadruplexes.
  • To highlight techniques for studying quadruplex structure, dynamics, and interactions.
  • To cover thermodynamic and kinetic analyses of quadruplexes and associated cations.

Main Methods:

  • Nuclear Magnetic Resonance (NMR) spectroscopy techniques.

More Related Videos

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

Related Experiment Videos

Last Updated: Jul 10, 2026

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

  • Methods for structural determination of quadruplexes in solution.
  • Techniques for assessing quadruplex dynamics and folding pathways.
  • Thermodynamic and kinetic analysis using NMR.
  • Investigation of cation interactions with quadruplexes.

    • Main Results:

    • NMR spectroscopy enables detailed characterization of quadruplexes.
    • A range of NMR methods are available for structural, dynamic, and thermodynamic studies.
    • Cation interactions significantly influence quadruplex behavior.
    • Kinetics of quadruplex formation and dissociation can be elucidated.

    Conclusions:

    • Solution NMR spectroscopy is indispensable for comprehensive quadruplex analysis.
    • The presented methods offer powerful tools for advancing quadruplex research.
    • Further application of these techniques will deepen our understanding of quadruplexes in biological systems.