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Updated: Jul 10, 2026
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
Published on: August 1, 2018
Correlation of estrogen sulfotransferase activity and proliferation in normal and carcinomatous human breast. A
Jorge Raul Pasqualini1, Gerard Samuel Chetrite
1Hormones and Cancer Research Unit, Institut de Puériculture et de Périnatalogie, 26 Boulevard Brune, 75014 Paris, France. jorge.pasqualini@wanadoo.fr
Background:
Sulfotransferases are present in normal and cancerous human breast tissues. The purpose of this article is to present a hypothetical correlation of sulfotransferase activity with proliferation in breast cancer.
Materials And Methods:
Sulfotransferases were evaluated in breast cancer cells by determining the transformation of non-conjugated estrogens to the sulfates. Proliferation was evaluated by the action on cell growth or the size of a transplanted tumor. The effect was obtained using the progestins: nomegestrol acetate, promegestone, and medrogestone, as well as tibolone and its metabolites at concentrations of 5 x 10(-5) to 5 x 10(-9) M.
Results:
A possible correlation of sulfotransferase activity stimulation and cell growth inhibition provoked by the various progestins used, or by tibolone and its metabolites was shown.
Conclusion:
It is suggested that the antiproliferative effect of these compounds could be related to the decrease of bioactive estradiol by the formation of its biologically inactive sulfate as a consequence of the stimulatory effect by the various progestins or tibolone on sulfotransferase activity.
