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Updated: Jul 10, 2026
Analysis of the Mitochondrial Density and Longitudinal Distribution in Rat Live-Skeletal Muscle Fibers by Confocal Microscopy
Published on: December 1, 2023
Confocal microscopy study of the distribution, content and activity of mitochondria during Paracentrotus lividus
Giovanni Morici1, Maria Agnello, Filippo Spagnolo
1Dipartimento di Biologia Cellulare e dello Sviluppo A. Monroy, Università degli Studi di Palermo, viale delle Scienze, ed. 16, 90128 Palermo, Italy.
Abstract:
In the present paper we applied confocal microscopy and fluorescence technologies for studying the distribution and the oxidative activity of sea urchin (Paracentrotus lividus) mitochondria during development, by in vivo incubating eggs and embryos with cell-permeant MitoTracker probes. We calculated, by a mathematical model, the intensity values, the variations of intensity, and the variation index of incorporated fluorochromes. Data demonstrate that mitochondrial mass does not change during development, whereas mitochondrial respiration increases. In addition, starting from 16 blastomeres stage, some regions of the embryo contain organelles more active in oxygen consumption.
