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Related Experiment Videos

Translation control of gene expression.

A Oppenheim1, S Altuvia, D Kornitzer

  • 1Department of Molecular Genetics, Hebrew University-Hadassah Medical School, Jerusalem, Israel.

Journal of Basic and Clinical Physiology and Pharmacology
|July 1, 1991
PubMed
Summary

Bacteriophage lambda cIII gene expression is controlled by mRNA structure. Mutations alter cIII mRNA conformation, affecting translation initiation and ribosomal binding, revealing a novel gene regulation mechanism.

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Area of Science:

  • Molecular Biology
  • Virology
  • Genetics

Background:

  • The bacteriophage lambda cIII gene product is a key regulator of the lysogenic pathway.
  • Understanding cIII mRNA structure-function relationships is crucial for deciphering its regulatory role.

Purpose of the Study:

  • To investigate the structure-function relationship of the cIII mRNA.
  • To elucidate the mechanisms controlling cIII gene expression.

Main Methods:

  • Utilized a set of cIII expression mutants.
  • Employed defined in vitro systems to analyze mRNA conformations and ribosomal subunit binding.
  • Localized the RNaseIII responsive element (RRE).

Main Results:

  • cIII mRNA exists in two conformations at equilibrium.

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  • Mutations causing overexpression favor a conformation (structure B) allowing efficient 30S ribosomal subunit binding.
  • Mutations preventing translation lead to an alternative conformation (structure A) that occludes the translation initiation region.

    • Conclusions:

    • Regulation of the equilibrium between cIII mRNA conformations controls gene expression.
    • The RNaseIII enzyme plays a role in this regulation, with its responsive element located in the cIII coding region.
    • The precise mechanism of RNaseIII involvement requires further investigation.