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Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

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Published on: May 4, 2018

Strontium can increase some osteoblasts without increasing hematopoietic stem cells.

Stefania Lymperi¹, Nicole Horwood, Stephen Marley

¹Stem Cell Biology Section, Kennedy Institute of Rheumatology and Division of Investigative Sciences, Hammersmith Hospital, Imperial College, London, United Kingdom.

|November 1, 2007

Summary

Strontium (Sr) increased osteoblast numbers but did not enhance hematopoietic stem cells (HSCs). N-cadherin positive osteoblasts, crucial for the HSC niche, were not affected, indicating their essential role in HSC regulation.

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Published on: February 16, 2024

Area of Science:

Bone Biology and Hematopoiesis
Stem Cell Niche Research

Background:

Osteoblasts regulate the hematopoietic stem cell (HSC) niche, influencing HSC numbers.
N-cadherin positive osteoblasts are specifically implicated as fundamental to the HSC niche.

Purpose of the Study:

To investigate the effect of strontium (Sr), a bone anabolic agent, on hematopoiesis and HSCs.
To determine if increasing overall osteoblast number and function can enhance HSC quantity and function.

Main Methods:

In vitro treatment of primary murine osteoblasts with Sr.
In vivo administration of Sr to mice, followed by analysis of bone parameters and HSCs.
HSC transplantation assays using Sr-treated mice as donors and recipients.

Main Results:

Sr treatment increased osteoblast number, bone volume, and bone nodule formation in vitro and in vivo.
Sr administration did not affect primitive HSC numbers but increased hematopoietic progenitors.
Sr treatment did not alter N-cadherin positive osteoblasts or transcripts.
HSC transplantation showed no difference in engraftment but delayed recovery when Sr-treated mice were recipients.

Conclusions:

Increasing osteoblast numbers and function with Sr is insufficient to enhance HSC quantity and function.
N-cadherin positive osteoblasts are essential for maintaining HSC quantity and function within the niche.
The study reinforces the critical role of N-cadherin(+) osteoblasts in the hematopoietic stem cell niche.