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Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Tandem Mass Spectrometry

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Updated: Jul 10, 2026

Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)

Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)

Published on: August 18, 2017

Mass spectrometry and peptide-based vaccine development.

I G Ovsyannikova¹, K L Johnson, H R Bergen

¹Mayo Vaccine Research Group, Mayo Clinic College of Medicine, Rochester, Minnesota, USA.

Clinical Pharmacology and Therapeutics

|November 1, 2007

Summary

Developing peptide-based vaccines is key for infectious disease control. Mass spectrometry advances aid in identifying pathogen T-cell epitopes for targeted vaccine design against viral and microbial threats.

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Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)

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Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

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Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

Area of Science:

Immunology
Vaccinology
Proteomics

Background:

Infectious disease control relies heavily on developing new vaccines.
Pathogen-derived peptides, including B-cell and T-cell epitopes, have been identified.
Synthetic peptide vaccines show promising results in early evaluations.

Purpose of the Study:

To review efforts in identifying T-cell epitopes for vaccine development.
To highlight the role of mass spectrometry in isolating and identifying pathogen peptides.
To discuss advancements in mass spectrometry for peptide sequencing.

Main Methods:

Characterization of pathogen-derived antigenic peptides.
Design and evaluation of synthetic peptide vaccine candidates.
Application of mass spectrometry for isolating and identifying pathogen peptides from human leukocyte antigen (HLA) molecules.

Main Results:

Significant improvements in mass spectrometer sensitivity and speed for peptide sequencing.
Enhanced accuracy in mass measurement for peptide identification.
Identification of T-cell epitopes crucial for directed vaccine development.

Conclusions:

Mass spectrometry is a pivotal tool in identifying T-cell epitopes for vaccine design.
Advancements in mass spectrometry accelerate the development of effective peptide-based vaccines.
Targeted vaccine development against viral and microbial pathogens is advancing through epitope identification.