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Updated: Jul 10, 2026
11:54
Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry
Published on: July 13, 2019
[Correlation between load of polyomavirus and hemorrhagic cystitis]
Chun-Rong Tong1, Zhi-Ping Teng, Hong-Xing Liu
1Beijing Dao-Pei Hospital, Beijing, China. cr.21@163.com
Summary
Polyomavirus BK virus (BKV) and Jamestown Canyon virus (JCV) are detectable in urine. Higher polyomavirus load correlates with increased risk of hemorrhagic cystitis after allogeneic stem cell transplantation.
Area of Science:
- Virology
- Oncology
- Immunology
Context:
- Allogeneic stem cell transplantation (SCT) is a life-saving procedure.
- Hemorrhagic cystitis (HC) is a serious complication post-SCT.
- Polyomaviruses, including BK virus (BKV) and JC virus (JCV), are implicated in HC.
Purpose:
- To investigate the correlation between polyomavirus load and HC incidence.
- To detect BKV, JCV, and SV40 VP1 genes in SCT patients and healthy individuals.
- To quantify viral load using polymerase chain reaction (PCR) and EvaGreen fluorescence assay.
Summary:
- BKV and JCV VP1 genes were detected in urine samples from healthy individuals and SCT patients.
- BKV was found in 100% of SCT patients' blood and urine.
- A significant correlation was observed between polyomavirus load and the occurrence of HC.
Impact:
- This study highlights the diagnostic significance of urinary polyomavirus detection.
- Findings may inform strategies for HC prevention and management in SCT recipients.
- Understanding polyomavirus dynamics is crucial for improving SCT outcomes.
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