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Related Experiment Video

Updated: Jul 10, 2026

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
10:23

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Published on: January 19, 2017

Single lymphocyte analysis with a microwell array chip.

Yoshiharu Tokimitsu1, Hiroyuki Kishi, Sachiko Kondo

  • 1Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|November 1, 2007
PubMed
Summary

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A novel cytomics system analyzes individual cell responses in large populations. This technology successfully generated human monoclonal antibodies against hepatitis B virus, paving the way for new therapeutics.

Area of Science:

  • Cellular biology
  • Immunology
  • Biotechnology

Background:

  • Cytomics is an emerging field for analyzing cellular behavior at the single-cell level.
  • Existing methods like flow cytometry have limitations in analyzing historical cellular responses.
  • High-throughput single-cell analysis is crucial for understanding complex biological processes.

Purpose of the Study:

  • To develop and validate a highly integrated live-cell microarray system for quantitative, single-cell analysis.
  • To demonstrate the system's capability in analyzing cellular responses and generating therapeutic antibodies.
  • To explore applications in infectious disease diagnosis and treatment.

Main Methods:

  • Development of a microwell array chip with 234,000 individual cell-sized microwells.

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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

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Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
10:31

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

Published on: April 8, 2016

Related Experiment Videos

Last Updated: Jul 10, 2026

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
10:23

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

Published on: January 19, 2017

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
10:31

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

Published on: April 8, 2016

  • Live-cell imaging and analysis of cellular responses within the microwell array.
  • Application of the system to isolate and analyze human antigen-specific B-cells.
  • Construction of a mouse model to assess antibody neutralization activity.

    • Main Results:

    • The system successfully analyzed cellular responses of individual cells in large populations.
    • Human monoclonal antibodies (MoAb) against hepatitis B virus surface antigen were generated.
    • Demonstrated neutralization activity of the developed antibodies in a mouse model.
    • The technology enables historical analysis of cellular responses, surpassing flow cytometry limitations.

    Conclusions:

    • The developed live-cell microarray system offers a powerful platform for advanced cytomic analysis.
    • This technology facilitates the generation of human monoclonal antibodies for therapeutic and diagnostic applications.
    • Potential impact on infectious disease research and treatment, particularly for viral infections like hepatitis B.