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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jul 10, 2026

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Published on: June 10, 2025

A method for measuring intracellular free magnesium concentration in platelets using flow cytometry.

Chester H Fox¹, Earl A Timm, Susan J Smith

¹University at Buffalo, The State University of New York, Department of Family Medicine, Family Medicine Research Institute, Buffalo, New York, USA. chetfox@gmail.com

Magnesium Research

|November 2, 2007

Summary

Researchers developed a new method to measure intracellular magnesium in platelets. This breakthrough allows for reliable assessment of magnesium levels, aiding the study of magnesium deficiency and related health conditions.

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Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies

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Last Updated: Jul 10, 2026

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Analyzing Platelet Subpopulations by Multi-color Flow Cytometry

Published on: June 10, 2025

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies

Published on: January 10, 2025

Area of Science:

Biochemistry
Cell Biology
Medical Diagnostics

Background:

Magnesium is crucial for over 302 enzymatic reactions and its deficiency is linked to insulin resistance, hypertension, diabetes, hyperlipidemia, and cardiovascular disease.
Studying magnesium deficiency is challenging due to magnesium's intracellular nature and measurement difficulties.

Purpose of the Study:

To develop a reproducible assay for measuring intracellular magnesium in human platelets.
To establish a reliable method for assessing magnesium status in clinical research.

Main Methods:

Developed a flow cytometry assay using Mag Green fluorescent dye to quantify intracellular free magnesium.
Utilized platelet-rich plasma from healthy volunteers and created standard titer curves for magnesium chloride.
Validated the assay with 15 adult participants, achieving high correlation (>0.99) for standard curves.

Main Results:

The assay demonstrated reliable and reproducible measurement of intracellular free magnesium in platelets.
The mean concentration of intracellular free magnesium was found to be 450.05 microM (range: 203.68–673.50 microM).

Conclusions:

Intracellular free magnesium in platelets can be accurately measured using Mag Green dye and flow cytometry.
This novel assay provides a valuable tool for advancing research into magnesium deficiency states and their health implications.