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[Study on HPLC fingerprint of SFE ginger extract]
1Henan Institute of Traditional Chinese medicine, Zhengzhou 450004, Henan. yandonghai963@yahoo.com.cn
Summary
A standardized High-Performance Liquid Chromatography (HPLC) method establishes a reliable fingerprint for supercritical fluid extraction (SFE) ginger extract, ensuring consistent quality control.
Area of Science:
- Pharmacognosy
- Analytical Chemistry
Context:
- Supercritical fluid extraction (SFE) is a key technology for obtaining bioactive compounds from ginger.
- Standardized quality control is essential for herbal medicines and extracts.
Purpose:
- To develop and validate a High-Performance Liquid Chromatography (HPLC) method for establishing a standard fingerprint of SFE ginger extract.
- To ensure the reproducibility and reliability of the analytical method.
Summary:
- An HPLC method utilizing an ODS column with a gradient mobile phase of acetonitrile and phosphoric acid was developed.
- Detection was performed at 280 nm with a column temperature of 25°C.
- The method demonstrated high similarity (>0.9) in precision, stability, and repeatability tests, confirming its robustness.
Impact:
- This validated HPLC method provides a reliable tool for the quality control of SFE ginger extract.
- It facilitates the consistent production and standardization of ginger-based products.
- Ensures the safety and efficacy of SFE ginger extract in various applications.
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01:03IR Frequency Region: Fingerprint Region
IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the C=O, C=N, and C=C occur between 1600–1850 cm−1.
The...
The...
01:18Supercritical Fluid Chromatography
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