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DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes
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DNA Electrophoresis Using Thiazole Orange Instead of Ethidium Bromide or Alternative Dyes

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pH-dependent fluorescence of uncharged benzothiazole-based dyes binding to DNA

Shuji Ikeda1, Akimitsu Okamoto

  • 1The Frontier Research System, RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama, 351-0198, Japan.

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology
|November 2, 2007
PubMed

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...