Factors influencing spontaneous mobilization of CD34+ and CD133+ progenitor cells after myocardial infarction
R G Turan1, M Brehm, M Koestering
1Laboratory of Stem Cell Transplantation and Molecular Cardiology, Division of Cardiology, Pneumology and Angiology, Heinrich-Heine-University, Moorenstrasse 6, Duesseldorf, Germany. turan@uni-duesseldorf.de
Insights
Mobilization of bone marrow-derived circulating progenitor cells (BM-CPCs) after acute myocardial infarction (AMI) is reduced by age, cardiovascular risk factors, and inflammation. This mobilization serves as a key indicator of heart function and regeneration potential.
Area of Science:
- Cardiovascular Medicine
- Regenerative Medicine
- Hematology
Background:
- Bone marrow-derived circulating progenitor cells (BM-CPCs) are mobilized into peripheral blood (PB) following acute myocardial infarction (AMI).
- These cells may play a role in regenerating damaged heart tissue after AMI.
- Understanding factors influencing BM-CPC mobilization is crucial for assessing cardiac recovery.
Purpose of the Study:
- To investigate the relationship between BM-CPC mobilization into PB and cardiovascular risk factors (CVRFs), patient age, inflammatory markers, and left ventricular function post-AMI.
- To identify key determinants of BM-CPC mobilization in the context of myocardial infarction.
Main Methods:
- Peripheral blood concentrations of CD34/45(+) and CD133/45(+) BM-CPCs were quantified using flow cytometry.
- The study included 44 patients post-AMI and 16 control subjects with atypical chest pain.
Main Results:
- BM-CPC mobilization on day 1 post-AMI negatively correlated with age, CVRFs, infarct size, and peak creatine phosphokinase.
- Elevated C-reactive protein and leukocyte levels were associated with decreased CD34/45(+) BM-CPC concentrations.
- BM-CPC concentrations increased significantly in AMI patients, peaking on day 7 compared to controls.
Conclusions:
- BM-CPC mobilization into PB post-AMI is influenced by CVRFs, age, infarct size, and inflammation.
- Circulatory dysfunction severity and necrotic myocardial tissue amount are primary determinants of BM-CPC mobilization.
- BM-CPC mobilization can serve as a surrogate marker for infarct size, global ejection fraction, and regenerative capacity post-AMI.
Background:
Bone marrow-derived circulating progenitor cells (BM-CPCs) are mobilized into adult peripheral blood (PB) during acute myocardial infarction (AMI) and may contribute to the regeneration of infarcted myocardium. The purpose of the present study is to determine whether mobilization of BM-CPCs into PB depends on cardiovascular risk factors (CVRFs), age of patients, infarct associated inflammatory markers, and left ventricular function after AMI.
Materials And Methods:
Peripheral blood concentrations of CD34/45(+) and CD133/45(+) BM-CPCs were measured by flow cytometry in 44 patients after AMI and in 16 subjects with atypical chest pain acting as controls.
Results:
Mobilization of CD34/45(+) and CD133/45(+) BM-CPCs on day 1 after AMI showed significant negative correlation with age, the number of CVRFs, infarct size, creatine phosphokinase peak in bivariate as well as in multivariate analyses. We additionally found a positive correlation of CD34/45(+) and CD133/45(+) BM-CPCs mobilization on day 1 after AMI with global ejection fraction (EF) in bivariate analysis but could not confirm this in multivariate analysis. Elevated of C-reactive protein (CRP) and leukocyte levels on day 1 after AMI were significantly associated with decreased concentrations of CD34/45(+) BM-CPCs. The concentrations of CD34/45(+) and CD133/45(+) BM-CPCs significantly increased in AMI patients, with the peak on day 7 as compared to the control group.
Conclusions:
The mobilization of CD34/45(+) and CD133/45(+) BM-CPCs into the PB depends on many factors, i.e. the number of CVRFs, age, infarct size and inflammatory markers of patients. Most importantly, the severity of the circulatory dysfunction and the amount of necrotic myocardial tissue are the main determinants. Moreover, this spontaneous mobilization of BM-CPCs may serve as a very important surrogate for infarct size as well as for global EF and it may determine the regenerative potency after AMI.
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