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Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens
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Cell mediated responses to the mycobacterium tuberculosis specific ESAT-6 antigen in Egyptian tuberculosis patients

Maha S Abd El-Maged1, Sahar A Shoman, Maged M Al-Sherbieny

  • 1Egyptian Organization for Biological Products and Vaccines (VACSERA Holding Company), Egypt.

The Egyptian Journal of Immunology
|November 3, 2007
PubMed

Human T cell responses and gamma interferon (IFN-gamma) secretion by peripheral blood mononuclear cells (PBMCs) in response to M. tuberculosis Early Secretory Antigen Target-6 (ESAT-6) eight synthetic overlapping peptides, were investigated in Egyptian tuberculosis patients as well as a control group. Three cell mediated immunoassays (lymphoproliferative, ELISPOT and intracellular flowcytometry) have been employed in this study to determine the ability of ESAT-6 to induce T-cell responses and IFN-gamma secretion, which play a critical role in protective cell-mediated immunity against tuberculosis (TB). The results revealed that all ESAT-6 peptides (P1-P8) were recognized by 85% of infected TB-patients, indicating that the molecule holds multiple epitopes. However, patients differed in the fine specificity of their peptide responses. Recognition of the N-terminal region of (P2-P7) was predominant. This study demonstrates that ESAT-6 is frequently recognized during infection and holds potential as a component of a future TB-vaccine.

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