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Related Experiment Video

Updated: Jul 10, 2026

Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly
10:18

Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly

Published on: February 9, 2015

An easy cloning and expression vector system for Gluconobacter oxydans.

Ute Schleyer1, Stephanie Bringer-Meyer, Hermann Sahm

  • 1Institut für Biotechnologie 1, Forschungszentrum Jülich GmbH, D-52425 Jülich, Germany. ute.schleyer@vetter-pharma.com

International Journal of Food Microbiology
|November 3, 2007
PubMed
Summary

Researchers developed the pEXGOX plasmid for efficient gene cloning in Gluconobacter oxydans. This new tool enhances the study of gene function in this important biotechnological microorganism.

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Area of Science:

  • Biotechnology
  • Microbiology
  • Molecular Biology

Background:

  • Gluconobacter oxydans is utilized in biotechnological processes for incomplete substrate oxidation.
  • Characterizing gene products is crucial for maximizing the oxidative capabilities of G. oxydans.

Purpose of the Study:

  • To construct a novel cloning and expression vector for G. oxydans.
  • To facilitate the study of gene function in G. oxydans.

Main Methods:

  • Development of the pEXGOX vector based on pBBR1MCS5.
  • Utilizing the genome sequence of G. oxydans DSM 2343.
  • Cloning G. oxydans genes into the pEXGOX vector.

Main Results:

  • The pEXGOX vector (5.7 kbp) offers a small size, complete sequence, unique restriction sites, direct PCR product cloning, and a strong promoter.

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Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1&#945; Promoter Using Gibson Assembly
10:18

Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly

Published on: February 9, 2015

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Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

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  • Successfully cloned G. oxydans genes using the pEXGOX plasmid.
  • Demonstrated increased cloning efficiency and decreased cloning duration.

    • Conclusions:

    • The pEXGOX vector is a valuable tool for G. oxydans research.
    • This vector has the potential to accelerate functional genomics studies in G. oxydans.
    • Facilitates understanding of G. oxydans open reading frames.