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Sexual Crosses with the Mucoromycete Phycomyces blakesleeanus

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Mutations in mating-type genes greatly decrease repeat-induced point mutation process in the fungus Podospora

Sylvie Arnaise¹, Denise Zickler, Anne Bourdais

¹Univ Paris-Sud, Institut de Génétique et Microbiologie, Bâtiment 400, UMR8621, F-91405 Orsay, France.

Fungal Genetics and Biology : FG & B

|November 6, 2007

Summary

Mating-type genes in filamentous fungi regulate Repeat-Induced point Mutation (RIP) and Premeiotic Recombination (PR). Mutations in these genes decrease RIP and PR frequencies, impacting DNA silencing.

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Area of Science:

Molecular Biology
Genetics
Mycology

Background:

Repeat-Induced point Mutation (RIP) and Premeiotic Recombination (PR) are key developmental processes in filamentous ascomycetes.
These DNA silencing mechanisms occur between fertilization and premeiotic replication.
Mating-type genes are known to influence fungal development, including fruiting body formation.

Purpose of the Study:

To investigate the role of mating-type genes in regulating RIP and PR frequencies.
To determine the correlation between the severity of mating-type gene mutations and their impact on RIP and PR.
To propose models explaining the interaction between mating-type genes and these DNA silencing processes.

Main Methods:

Comparative analysis of RIP and PR frequencies in wild-type and mutant strains.
Assessment of mating-type gene alleles with varying effects on fruiting-body development.
Genetic analysis to establish correlations between gene function and silencing efficiencies.

Main Results:

Mutations in mating-type genes significantly reduce the frequencies of both RIP and PR.
Alleles with a stronger impact on fruiting-body development exhibit a more pronounced reduction in RIP and PR.
A clear correlation exists between mating-type gene function and the efficiency of these DNA silencing pathways.

Conclusions:

Mating-type genes play a crucial role in modulating RIP and PR frequencies in filamentous ascomycetes.
Two models are proposed: direct regulation by mating-type proteins or indirect regulation via developmental control.
Understanding this relationship provides insights into genome stability and fungal development.