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Group A rotavirus-associated diarrhea in children seeking treatment in Indonesia

Shannon D Putnam1, Endang R Sedyaningsih, Erlin Listiyaningsih

  • 1U.S. Naval Medical Research Unit No. 2, Jakarta, Indonesia. putnam@namrutwo.org

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
|November 6, 2007
PubMed

Insights

Rotavirus is a major cause of childhood diarrhea in Indonesia, with prevalence at 45.5%. Genotyping revealed a shift to G9P[8], suggesting a multivalent vaccine could reduce illness burden.

Area of Science:

  • Pediatrics
  • Infectious Diseases
  • Virology

Background:

  • Group A rotavirus is a leading cause of severe diarrhea and death in children globally.
  • Epidemiological data on rotavirus disease in Indonesian children is scarce.

Purpose of the Study:

  • To investigate the epidemiology of rotavirus-associated diarrhea in Indonesian children under six years old.
  • To identify clinical symptoms and prevalent rotavirus genotypes.

Main Methods:

  • A hospital-based case series study was conducted across four referral hospitals from February 2004 to February 2005.
  • Rotavirus detection utilized enzyme immunoassay (EIA) and reverse transcription-polymerase chain reaction (RT-PCR).
  • Specific rotavirus genotypes were determined using semi-nested RT-PCR.

Main Results:

  • Out of 1660 stool samples tested, 45.5% were positive for rotavirus.
  • Children with rotavirus diarrhea were younger and more likely to be hospitalized.
  • Common symptoms included vomiting, fever, nausea, fatigue, and dehydration; bloody stools were less frequent.

Conclusions:

  • Rotavirus significantly contributed to morbidity in the study population.
  • A temporal shift in rotavirus genotypes from G1-G4 to G9 was observed, strongly associated with the P[8] gene.
  • A multivalent rotavirus vaccine targeting G9P[8] may decrease the burden of diarrheal diseases in Indonesian children.
Abstract

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