Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Fluid Movement Between Compartments

Fluid Movement Between Compartments

The force applied by fluids against a surface, known as hydrostatic pressure, initiates the transfer of fluid among different compartments. Within our blood vessels, the blood's hydrostatic pressure is a result of the heart's pumping action. At the arteriolar end of capillaries, hydrostatic pressure (capillary blood pressure) exceeds the opposing colloid osmotic pressure created primarily by plasma proteins like albumin. This discrepancy in pressure propels plasma and nutrients from the...

Blood Pressure

Blood Pressure

The movement of blood in a human body, commonly referred to as blood flow, is determined by the volume of blood that traverses a certain section of the bodily system per unit time. It is the rhythmic contraction of the heart's ventricles that primarily instigates this movement. As the ventricles contract, blood is forced into the prominent arteries, which then flow from areas of greater pressure to lower pressure areas. This movement continues into smaller arteries and arterioles and...

Overview of the Vascular System

Overview of the Vascular System

The vascular system comprises an extensive network of arteries, capillaries, and veins. The vascular system can be broadly divided into the blood and lymphatic systems. Typically, blood vessels can be categorized into three histological regions: tunica intima, tunica media, and tunica adventitia. The tunica intima consists of a single layer of endothelial cells attached to the basal lamina. Underlying the basal lamina is a connective tissue layer and an elastic lamina that gives stability and...

Physiological Barriers

Physiological Barriers

Physiological barriers are semi-permeable cellular structures restricting drug diffusion into intracellular compartments and tissues. There are six types of physiological barriers: blood endothelial, cell membrane, blood-brain, blood-cerebrospinal fluid (CSF), blood-placenta, and blood-testis barriers.
The blood endothelial barrier is the most porous of these. It allows all small ionized, un-ionized, and lipophilic molecules to pass through the endothelial lining into the interstitial space...

Pressure of Fluids

Pressure of Fluids

There are many examples of pressure in fluids in everyday life, such as in relation to blood (high or low blood pressure) and in relation to weather (high- and low-pressure weather systems). A given force can have a significantly different effect, depending on the area over which the force is exerted. For instance, a force applied to an area of 1 mm2 has a pressure that is 100 times greater than the same force applied to an area of 1 cm2. That's why a sharp needle is able to poke through skin...

Glomerular Filtration: Net Filtration Pressure

Glomerular Filtration: Net Filtration Pressure

Glomerular filtration, a key process in the kidneys, is regulated by three main pressures: Glomerular blood hydrostatic pressure (GBHP), Capsular hydrostatic pressure (CHP), and Blood colloid osmotic pressure (BCOP).
GBHP, with an average value of 55 mmHg, promotes filtration by pushing water and solutes through the filtration membrane. This is balanced by two opposing forces: CHP, a "back pressure" exerted against the filtration membrane by fluid already in the capsular space and renal tubule,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Visualization of Pulpal Structures by SWIR in Endodontic Access Preparation.

Journal of dental research·2024

Same author

Ca<sup>2+</sup> signalling is critical for autoantibody-induced blistering of human epidermis in pemphigus.

The British journal of dermatology·2021

Same author

A new ex vivo human oral mucosa model reveals that p38MAPK inhibition is not effective in preventing autoantibody-induced mucosal blistering in pemphigus.

The British journal of dermatology·2019

Same author

Inhibition of p38MAPK signalling prevents epidermal blistering and alterations of desmosome structure induced by pemphigus autoantibodies in human epidermis.

The British journal of dermatology·2017

Same author

Mind the gap: mechanisms regulating the endothelial barrier.

Acta physiologica (Oxford, England)·2017

Same author

Epac1 - a tonic stabilizer of the endothelial barrier.

Acta physiologica (Oxford, England)·2016

Search research articles

Related Experiment Video

Updated: Jul 10, 2026

Imaging Leukocyte Adhesion to the Vascular Endothelium at High Intraluminal Pressure

Imaging Leukocyte Adhesion to the Vascular Endothelium at High Intraluminal Pressure

Published on: August 23, 2011

Physiological hydrostatic pressure protects endothelial monolayer integrity.

K Müller-Marschhausen¹, J Waschke, D Drenckhahn

¹Institute of Anatomy and Cell Biology, University of Würzburg, Würzburg, Germany.

American Journal of Physiology. Cell Physiology

|November 6, 2007

Summary

Physiological hydrostatic pressure protects endothelial monolayer integrity by maintaining vascular-endothelial cadherin at cell junctions. This protective effect involves caveolae-dependent mechanisms, crucial for endothelial barrier function in various conditions.

Related Experiment Videos

Last Updated: Jul 10, 2026

Imaging Leukocyte Adhesion to the Vascular Endothelium at High Intraluminal Pressure

Imaging Leukocyte Adhesion to the Vascular Endothelium at High Intraluminal Pressure

Published on: August 23, 2011

Area of Science:

Cardiovascular Biology
Cell Biology
Biophysics

Background:

Endothelial monolayer integrity is vital for barrier function and is compromised in diseases like atherosclerosis.
Endothelial cells experience mechanical forces in vivo, but the impact of hydrostatic pressure remains understudied.

Purpose of the Study:

To investigate the hypothesis that physiological hydrostatic pressure protects endothelial monolayer integrity in vitro.
To elucidate the mechanisms underlying hydrostatic pressure's effects on endothelial cells.

Main Methods:

Utilized microvascular myocardial endothelial (MyEnd) cells and macrovascular pulmonary artery endothelial cells (PAECs).
Applied pharmacological agents (EGTA, cytochalasin D, trifluperazine, thrombin) to disrupt monolayer integrity with and without hydrostatic pressure (15 cmH2O).
Employed laser tweezer trapping to quantify VE-cadherin-mediated adhesion and studied caveolin-1-deficient cells.

Main Results:

Hydrostatic pressure prevented EGTA-induced loss of vascular-endothelial cadherin (VE-cadherin) at cell junctions.
Pressure reduced cytochalasin D-induced actin depolymerization, gap formation, and cell detachment.
Thrombin-induced cell dissociation was also attenuated by hydrostatic pressure.
Hydrostatic pressure protected VE-cadherin-mediated adhesion in MyEnd cells, an effect absent in caveolin-1-deficient cells.

Conclusions:

Physiological hydrostatic pressure protects endothelial monolayer integrity and barrier function.
Caveolae-dependent mechanisms are essential for sensing hydrostatic pressure and mediating its protective effects.
Findings highlight the role of mechanical forces in maintaining endothelial health and suggest therapeutic potential.