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Updated: Jul 10, 2026

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
Published on: May 5, 2023
Actinocatenispora sera sp. nov., isolated by long-term culturing
Atsuko Matsumoto1, Yōko Takahashi2, Megumi Fukumoto2
1The Kitasato Institute, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8642, Japan.
Abstract:
Two novel actinomycete strains, KV-744T and KV-856, were isolated by long-term cultivation. Aerial long-chain spores were produced directly from vegetative mycelia and possessed no motility. Vegetative mycelia developed very well and exhibited fragmentation. The cell-wall peptidoglycan contained meso-diaminopimelic acid, glycine, alanine and glutamic acid, and whole-cell hydrolysates contained arabinose, galactose and xylose. The acyl type of the peptidoglycan was glycolyl. The predominant menaquinone was MK-9(H4) and mycolic acids were not detected. The diagnostic phospholipid was phosphatidylethanolamine. The predominant cellular fatty acids were 14-methylhexadecanoic (ai-C17:0), 14-methylpentadecanoic (i-C16:0), 15-methylhexadecanoic (i-C17:0) and 13-methyltetradecanoic (i-C15:0) acids. The G+C content of the DNA was 72-73 mol%. The phenotypic and chemical properties indicated that the two isolates belong to the family Micromonosporaceae and the 16S rRNA gene sequence analysis suggested that the closest relationship was with Actinocatenispora thailandica. The DNA-DNA hybridization values between strain KV-744T or KV-856 and A. thailandica TT2-10T were 42-53%. Based on the data above, strains KV-744T and KV-856 should be classified as representing a novel species of the genus Actinocatenispora, for which the name Actinocatenispora sera sp. nov. is proposed. The type strain is KV-744T (=NRRL B-24477T=NBRC 101916T).
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