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Rapid determination of succinylcholine in human plasma by high-performance liquid chromatography with fluorescence
A J Lagerwerf1, L E Vanlinthout, T B Vree
1Department of Anaesthesiology, Academic Hospital Nijmegen Sint Radboud, Netherlands.
Abstract:
A high-performance liquid chromatographic method with fluorometric detection has been developed for the determination of succinylcholine in human plasma. Succinylcholine shows fluorescence at 282 nm with an excitation at 257 nm. The assay is sensitive, reproducible and linear for concentrations ranging from 100 ng/ml to 100 micrograms/ml of succinylcholine. In a pilot study the plasma concentration-time curve showed a triphasic elimination, with half-lives of 0.4, 1.2 and 8 min, respectively. In a clinical setting, drugs commonly administered during anaesthesia did not interfere with the assay. This method provides a simple and time-saving alternative to existing methods.