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Sanguinarine levels in biological samples by high-performance liquid chromatography
P Reinhart1, R Harkrader, R Wylie
1ATRIX Laboratories, Inc., Ft. Collins, CO 80522-2501.
Journal of Chromatography
|October 4, 1991
Summary
A new high-performance liquid chromatography method accurately detects sanguinarine in plant extracts and biological samples like saliva. This technique offers a sensitive detection limit for analyzing the benzophenanthridine alkaloid.
Area of Science:
- Analytical Chemistry
- Pharmacognosy
- Biochemistry
Background:
- Sanguinarine is a benzophenanthridine alkaloid found in plant extracts.
- Accurate quantification of sanguinarine is important for various applications.
- Existing analytical methods may require complex sample preparation.
Purpose of the Study:
- To develop and validate a high-performance liquid chromatographic (HPLC) method for sanguinarine analysis.
- To demonstrate the method's applicability to biological samples.
- To establish a sensitive and reliable quantification technique.
Main Methods:
- High-performance liquid chromatography (HPLC) using an ethyl silane column.
- Mobile phase optimization with acidic and basic ion-pairing reagents.
- Acidified methanolic extraction for sample preparation.
Main Results:
- The developed HPLC method successfully analyzed sanguinarine in plant extracts.
- The method was effective for analyzing sanguinarine in saliva and gingival crevicular fluid.
- A limit of detection of 3 ng of sanguinarine was achieved.
Conclusions:
- A robust and sensitive HPLC method for sanguinarine quantification has been established.
- The method is suitable for analyzing sanguinarine in diverse sample matrices.
- This technique facilitates the study of sanguinarine in biological and botanical contexts.