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Enantiospecific analysis of ketoprofen in plasma by high-performance liquid chromatography
P J Hayball1, R L Nation, F Bochner
1Pharmacy Department, Repatriation General Hospital, Daw Park, SA, Australia.
Journal of Chromatography
|October 4, 1991
Abstract:
A high-performance liquid chromatographic (HPLC) assay for the determination of the R- and S-enantiomers of ketoprofen is described. Facile ketoprofen extraction from plasma and derivatization to the diastereomeric S-1-phenylethylamides was followed by normal-phase HPLC. The ketoprofen diastereomeric amides eluted within 8 min. The limit of quantification of the assay was 0.15 mg/l of each enantiomer (signal-to-noise ratio = 5).