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Multi-species Conserved Sequences

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...

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Updated: Jul 10, 2026

Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate

Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate

Published on: September 18, 2013

Sequence conservation between porcine and human LRRK2.

Knud Larsen¹, Lone Bruhn Madsen

¹Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, University of Aarhus, Blichers Alle 20, P.O. Box 50, 8830 Tjele, Denmark. Knud.Larsen@agrsci.dk

Molecular Biology Reports

|November 6, 2007

Summary

This summary is machine-generated.

Researchers cloned the porcine Leucine-rich repeat kinase 2 (LRRK2) gene, finding conserved sequences and expression patterns similar to humans. This suggests LRRK2 is a potential target for developing Parkinson

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Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

Published on: March 21, 2020

Area of Science:

Genetics
Neuroscience
Molecular Biology

Background:

Leucine-rich repeat kinase 2 (LRRK2) is a ROCO protein superfamily member.
LRRK2 gene mutations are linked to autosomal dominant Parkinsonism.

Purpose of the Study:

To clone porcine LRRK2 cDNA and characterize conserved functional domains.
To investigate the potential of porcine LRRK2 in Parkinson's disease modeling.

Main Methods:

Cloning of porcine LRRK2 cDNA.
Sequence homology analysis with mammalian counterparts.
Porcine LRRK2 mRNA expression profiling in various tissues.
Mapping the porcine LRRK2 gene to chromosome 5q25.

Main Results:

The porcine LRRK2 cDNA contains a 7,578 bp open reading frame, encoding a 2,526 amino acid protein.
Porcine LRRK2 exhibits 86-93% amino acid identity with mammalian counterparts, particularly in kinase, Roc, and COR domains.
LRRK2 mRNA expression is conserved across species and not restricted to the brain.
The porcine LRRK2 gene was mapped to chromosome 5q25.

Conclusions:

Porcine LRRK2 sequence and expression patterns are highly conserved with human orthologs.
The conserved nature of LRRK2 suggests its utility in studying Parkinson's disease.
The study supports the potential for generating a transgenic porcine model for Parkinson's disease using the LRRK2 gene.