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Related Experiment Video

Updated: Jul 10, 2026

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures
12:58

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures

Published on: March 26, 2019

Many-photon dynamics of photobleaching.

S Gavrilyuk1, S Polyutov, P C Jha

  • 1Theoretical Chemistry, Royal Institute of Technology, Roslagstullsbacken 15, S-106 91, Stockholm, Sweden. gavri@theochem.kth.se

The Journal of Physical Chemistry. A
|November 6, 2007
PubMed
Summary

This study presents a dynamical theory for photobleaching using laser pulses, explaining experimental results in pyrylium salts. It reveals two competitive photobleaching channels with distinct intensity dependencies, crucial for understanding material behavior.

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Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching
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Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching

Published on: February 26, 2010

Area of Science:

  • Photophysics and Photochemistry
  • Laser Spectroscopy
  • Materials Science

Background:

  • Photobleaching is a critical phenomenon in materials exposed to light.
  • Understanding photobleaching dynamics is essential for applications like optical data storage.
  • Pyrylium salts exhibit complex photophysical behavior under laser irradiation.

Purpose of the Study:

  • To develop a detailed dynamical theory for photobleaching induced by pulsed lasers.
  • To interpret experimental observations of photobleaching in pyrylium salts.
  • To elucidate the mechanisms and intensity dependencies of competing photobleaching pathways.

Main Methods:

  • First-principles simulations of photoabsorption cross-sections.
  • Utilization of empirical rate constants for dynamical modeling.
  • Analysis of two-photon and one-photon excitation processes.
  • Modeling of fluorescence dynamics and intensity dependencies.

Main Results:

  • Identified two competitive photobleaching channels: one from lower excited states and another from higher excited states.
  • Observed distinct intensity dependencies: quadratic for the lower-state channel and cubic for the higher-state channel.
  • Demonstrated that fluorescence decay follows double-exponential dynamics, attributed to ground-triplet state equilibrium and photobleaching.
  • Found photobleaching rates to be sensitive to laser parameters (repetition rate, pulse duration, peak intensity) and rate constants.

Conclusions:

  • The developed theory accurately explains experimental photobleaching data in pyrylium salts.
  • The interplay between different photobleaching channels dictates the overall material response.
  • Many-photon-induced photobleaching holds potential for developing novel 3D optical read-write devices.