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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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Related Experiment Video

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Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
10:50

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Published on: February 25, 2017

Direct quantification of gene expression using fluorescence correlation spectroscopy.

Yasutomo Nomura1, Takao Nakamura, Zhonggang Feng

  • 1Department of Environmental Life Science, Graduate School of Medical Science, Yamagata University, Yonezawa, Yamagata 992-8510, Japan. ynomura@yz.yamagata-u.ac.jp

Current Pharmaceutical Biotechnology
|November 6, 2007
PubMed
Summary
This summary is machine-generated.

Fluorescence Correlation Spectroscopy (FCS) is a powerful tool for single molecule detection in medicinal chemistry. This method quantifies molecules and molecular weight, aiding gene expression and interaction studies in living cells.

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Area of Science:

  • Medicinal Chemistry
  • Biophysics
  • Molecular Biology

Background:

  • Single molecule detection is crucial in medicinal chemistry.
  • Traditional methods often require physical separation, limiting real-time analysis.
  • Fluorescence Correlation Spectroscopy (FCS) offers a non-invasive alternative.

Purpose of the Study:

  • To highlight the growing importance and applications of FCS in medicinal chemistry.
  • To demonstrate FCS's utility in quantitative analysis of molecular interactions and gene expression.
  • To explore FCS's potential in studying mRNA transcription and related cellular processes.

Main Methods:

  • Utilizes Fluorescence Correlation Spectroscopy (FCS) for single molecule detection.
  • Measures the number of fluorescent molecules and diffusion constants related to molecular weight.
  • Does not require physical separation techniques like gel electrophoresis.

Main Results:

  • FCS enables direct quantification of fluorescently labeled molecules.
  • The technique allows for determination of molecular weight-independent diffusion characteristics.
  • FCS is suitable for studying oligonucleotide hybridization, gene expression, and protein translation.

Conclusions:

  • FCS is an advantageous method for molecular studies in medicinal chemistry.
  • Technical advancements in FCS expand its application to SNP analysis, transcription factor interactions, and real-time gene expression monitoring.
  • FCS facilitates quantitative analysis of mRNA in living cells, advancing research in molecular biology and drug discovery.