Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tumor necrosis factor-alpha and basic fibroblast growth factor decrease glial fibrillary acidic protein and its encoding mRNA in astrocyte cultures and glioblastoma cells.

Journal of neurochemistry·1995
Same author

Bile-independent absorption of cyclosporine from a microemulsion formulation in liver transplant patients.

Transplantation·1995
Same author

Expression of interleukin-11 and its encoding mRNA by glioblastoma cells.

Neuroscience letters·1995
Same author

Structural analysis of porcine brain nitric oxide synthase reveals a role for tetrahydrobiopterin and L-arginine in the formation of an SDS-resistant dimer.

The EMBO journal·1995
Same author

Potent and selective inhibition of nitric oxide-sensitive guanylyl cyclase by 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one.

Molecular pharmacology·1995
Same author

[Quality comparison of modified neurolept-, balanced and intravenous anesthesia. 1. Study design and patient analysis of the Krefelder study 1992].

Der Anaesthesist·1995

Related Experiment Video

Updated: Jul 10, 2026

NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode
09:19

NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode

Published on: June 4, 2021

SPR-based fragment screening: advantages and applications.

T Neumann1, H-D Junker, K Schmidt

  • 1Graffinity Pharmaceuticals, Im Neuenheimer Feld 518-519, 69120 Heidelberg, Germany.

Current Topics in Medicinal Chemistry
|November 6, 2007
PubMed
Summary

Surface Plasmon Resonance (SPR) is a powerful, label-free biophysical technique for drug discovery. SPR imaging with chemical microarrays enables high-throughput screening of large fragment libraries, identifying selective inhibitors for drug targets.

More Related Videos

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin
06:29

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin

Published on: March 3, 2021

Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source
08:35

Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source

Published on: May 29, 2021

Related Experiment Videos

Last Updated: Jul 10, 2026

NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode
09:19

NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode

Published on: June 4, 2021

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin
06:29

Workflow and Tools for Crystallographic Fragment Screening at the Helmholtz-Zentrum Berlin

Published on: March 3, 2021

Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source
08:35

Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source

Published on: May 29, 2021

Area of Science:

  • Biophysics
  • Drug Discovery
  • Chemical Biology

Background:

  • Fragment-based screening is a key strategy in modern drug discovery.
  • Various biophysical methods, including X-ray, NMR, and tethering, are used for fragment screening.
  • Surface Plasmon Resonance (SPR) offers sensitive, label-free detection of biomolecular interactions.

Purpose of the Study:

  • To detail the application of SPR techniques for primary screening of large fragment libraries in drug discovery.
  • To review the advantages of SPR over traditional assays and its use in biosensing.
  • To highlight the potential of SPR imaging with chemical microarrays for high-throughput fragment screening.

Main Methods:

  • Utilizing Surface Plasmon Resonance (SPR) for label-free detection of molecular interactions.
  • Employing SPR imaging combined with chemical microarrays for simultaneous affinity measurements.
  • Screening large fragment libraries (up to 110,000 compounds) using high-throughput SPR systems.

Main Results:

  • SPR imaging on chemical microarrays can analyze up to 9,216 immobilized fragments per array.
  • This high-throughput approach is suitable for screening extensive fragment libraries.
  • Case studies demonstrate successful identification of selective low molecular weight inhibitors.

Conclusions:

  • SPR, particularly SPR imaging with microarrays, significantly enhances the throughput of fragment-based drug discovery.
  • This method facilitates the identification of potent and selective inhibitors for various drug targets.
  • Optimized fragment library design, immobilization, and follow-up strategies are crucial for successful hit identification.