Related Experiment Video
Updated: Jul 10, 2026
06:34
A Tailored HPLC Purification Protocol That Yields High-purity Amyloid Beta 42 and Amyloid Beta 40 Peptides, Capable of Oligomer Formation
Published on: March 27, 2017
Expression and purification of uniformly (15)N-labeled amyloid beta peptide 1-40 in Escherichia coli
M Nagata-Uchiyama1, M Yaguchi, Y Hirano
1Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashiku, Fukuoka, Japan.
Protein and Peptide Letters
|November 6, 2007
Abstract:
For biophysical studies using heteronuclear NMR analysis of amyloid beta peptide, construction of an efficient and high yield expression system of uniformly isotopic labeled amyloid beta peptide is desirable. Here we succeeded in obtaining (15)N-labeled amyloid beta 1-40 expressed by attachment to hen egg white lysozyme as a fusion protein.
