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Expression, purification and characterisation of Haemophilus influenzae 3-isopropylmalate dehydrogenase (LeuB)
Sara Martignon1, Franca Rossi, Menico Rizzi
1DiSCAFF, University of Piemonte Orientale Amedeo Avogadro, Via Bovio 6, 28100, Novara, Italy.
Abstract:
3-isopropylmalate dehydrogenase (3IPMDH) is the third enzyme in leucine biosynthesis and a promising target for the development of broad-spectrum antibacterial agents. We report here the expression, purification and biochemical characterisation of Haemophilus influenzae 3-isopropylmalate dehydrogenase. The observed enzyme inhibition by the reaction product NADH could represent a regulatory mechanism for 3IPMDH.
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