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Properties of ionic currents induced by external ATP in a mouse mesodermal stem cell line
1Department of Neurophysiology, Tokyo Metropolitan Institute for Neurosciences, Japan.
The Journal of Physiology
|October 1, 1991
Summary
Adenosine triphosphate (ATP) activates calcium-dependent potassium channels in mesodermal stem cells, leading to hyperpolarization. This ATP-induced calcium increase and subsequent potassium current are key to cell signaling.
Area of Science:
- Cellular physiology
- Stem cell biology
- Ion channel research
Background:
- Mesodermal stem cells (C3H10T1/2) are crucial for tissue development.
- Adenosine triphosphate (ATP) is a key signaling molecule in various cell types.
- Understanding ATP's role in stem cell function is vital for regenerative medicine.
Purpose of the Study:
- To investigate the effects of ATP on ion channel activity in mesodermal stem cells.
- To characterize the specific ion currents induced by ATP.
- To elucidate the underlying mechanisms of ATP-induced cellular responses.
Main Methods:
- Whole-cell patch clamp recording to measure ion currents.
- Application of ATP and its analogues to C3H10T1/2 cells.
- Intracellular calcium measurements using Fluo-3.
- Pharmacological manipulation with ion channel blockers and activators.
Main Results:
- ATP induced slow outward potassium (K+) currents in 91% of cells and chloride (Cl-) currents in 10%.
- ATP-induced K+ current was dose-dependent (Kd=0.4 μM) and mediated by increased intracellular calcium.
- The Ca2+-activated K+ conductance resulted in hyperpolarization, with single-channel conductance estimated at 2.7 pS.
Conclusions:
- ATP increases intracellular calcium concentration in mesodermal stem cells.
- This calcium increase activates a Ca2+-activated K+ conductance, leading to hyperpolarization.
- ATP plays a significant role in regulating the membrane potential and signaling pathways in these stem cells.