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A novel medium devoid of ruminant peptone for high yield growth of Mycoplasma ovipneumoniae
Hiren Patel1, David Mackintosh, Roger D Ayling
1School of Life Sciences, Kingston University, Penrhyn Road, Kingston-Upon-Thames, Surrey KT1 2EE, UK.
Abstract:
Mycoplasma ovipneumoniae is considered an emerging veterinary pathogen causing pneumonia in sheep and goats worldwide. Currently it has not been possible to define a growth medium that yields the maximum growth of M. ovipneumoniae within a short incubation period. Growth yields of M. ovipneumoniae in Eaton's medium are variable and not as consistently high as those seen with other Mycoplasma spp. This study investigated the ability of different M. ovipneumoniae field strains to grow in various media formulations, where PPLO broth was replaced by a vegetable protein source, and comparisons were made in terms of strain viability in Eaton's medium. Studies were also conducted to determine the optimal carbohydrate source for use in the M. ovipneumoniae medium. Generally, it was found that different strains showed good growth in all media tested, with growth yields at 24h in TSB-1 medium higher than those observed with Eaton's medium. Growth yields reached 10(8) to 10(9)cfu ml(-1) within 24h for particular field strains, with all strains achieving this growth level within 48-72h.
Insights
Optimizing growth media for Mycoplasma ovipneumoniae, a key veterinary pathogen, is crucial. This study found a new TSB-1 medium supports faster and higher M. ovipneumoniae growth than Eaton's medium.
Area of Science:
- Veterinary Microbiology
- Animal Pathology
Background:
- Mycoplasma ovipneumoniae is an emerging veterinary pathogen causing significant pneumonia in sheep and goats globally.
- Current growth media for M. ovipneumoniae, such as Eaton's medium, exhibit variable and suboptimal growth yields.
- A need exists for improved culture media to enhance M. ovipneumoniae growth rates and yields for research and diagnostics.
Purpose of the Study:
- To investigate the growth performance of various M. ovipneumoniae field strains in novel media formulations.
- To compare the efficacy of a new medium (TSB-1) against Eaton's medium for M. ovipneumoniae cultivation.
- To identify optimal carbohydrate sources for maximizing M. ovipneumoniae growth.
Main Methods:
- Evaluated growth of different M. ovipneumoniae field strains in various media, including formulations with vegetable protein replacing PPLO broth.
- Compared strain viability and growth yields in TSB-1 medium versus Eaton's medium.
- Assessed the impact of different carbohydrate sources on M. ovipneumoniae growth.
Main Results:
- Most M. ovipneumoniae field strains demonstrated good growth across all tested media.
- TSB-1 medium consistently yielded higher growth rates at 24 hours compared to Eaton's medium.
- Specific field strains achieved 10(8) to 10(9) CFU/mL within 24 hours in TSB-1, with all strains reaching this level within 48-72 hours.
Conclusions:
- The TSB-1 medium formulation, utilizing a vegetable protein source, significantly enhances M. ovipneumoniae growth compared to Eaton's medium.
- This optimized medium facilitates rapid cultivation of M. ovipneumoniae field strains, crucial for advancing veterinary diagnostics and research.
- Further studies can explore specific carbohydrate sources to further refine M. ovipneumoniae culture conditions.
