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Updated: Jul 10, 2026

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis
Published on: March 9, 2018
A retrospective study on the coexistence of hydatid cyst and aspergillosis
Nazim Emrah Koçer1, Yasemin Kibar, Muhammed Emin Güldür
1Department of Pathology, Faculty of Medicine, Baskent University, Ankara, Turkey. nemrahkocer@yahoo.com
Objectives:
Hydatid cyst is a zoonotic disease with an endemic regional distribution, and Aspergillus is a saprophytic fungus that may cause allergic pulmonary aspergillosis, aspergilloma, and semi-invasive and invasive aspergillosis. The coexistence of a saprophytic fungus and hydatid cyst is extremely rare. The aim of this retrospective study was to evaluate the coexistence of aspergillosis and echinococcosis in archival materials and to discuss its probable clinical significance.
Methods:
Hematoxylin-eosin (HE)-stained sections of 100 archival cases with the diagnosis of hydatid cyst were reevaluated by four pathologists independently. Grocott's methenamine-silver (GMS) and periodic acid-Schiff (PAS) were applied to the slides that were suspected of having co-infection with Aspergillus to confirm the diagnosis.
Results:
Two cases of aspergillosis and hydatid cyst coexistence were found out of the 100 reevaluated archival cases with a diagnosis of hydatid cyst. Both of the cases were located in the lung, in immunocompetent patients.
Conclusions:
Aspergillosis and hydatid cyst coexistence may be important in patients with immune deficiency and in cases with pre- or perioperatively ruptured cysts. There are no reliable data on the specificity and sensitivity of radiological imaging techniques in detecting the existence of Aspergillus in hydatid cysts. Histopathological evaluation is essential for diagnosis and for the planning of management.
Insights
This study found a rare co-infection of aspergillosis and hydatid cyst in two lung cases. Histopathology is crucial for diagnosing this rare co-occurrence, especially in immunocompromised patients.
Area of Science:
- Medical Mycology
- Parasitology
- Pathology
Background:
- Hydatid cyst is an endemic zoonotic disease.
- Aspergillosis, caused by Aspergillus fungus, presents various lung pathologies.
- The co-infection of hydatid cyst and aspergillosis is exceptionally rare.
Purpose of the Study:
- To investigate the coexistence of aspergillosis and echinococcosis in archival materials.
- To discuss the potential clinical significance of this rare co-infection.
Main Methods:
- Reevaluation of 100 archival hydatid cyst cases by four pathologists.
- Application of Grocott's methenamine-silver (GMS) and periodic acid-Schiff (PAS) stains for suspected co-infections.
Main Results:
- Two cases of coexisting aspergillosis and hydatid cyst were identified among 100 cases.
- Both identified cases were located in the lung and occurred in immunocompetent patients.
Conclusions:
- The coexistence of aspergillosis and hydatid cyst warrants attention, particularly in immunocompromised individuals or with cyst rupture.
- Radiological imaging lacks reliable data for detecting Aspergillus within hydatid cysts.
- Histopathological examination is essential for accurate diagnosis and management planning.
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