Related Experiment Video

Updated: Jul 10, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Pre-mRNA splicing aberrations and cancer

Christopher A Pettigrew1, Melissa A Brown

  • 1School of Molecular and Microbial Sciences, University of Queensland, St. Lucia, Queensland, Australia, 4072.

Frontiers in Bioscience : a Journal and Virtual Library
|November 6, 2007
PubMed

Insights

Accurate gene splicing is crucial for proper protein production. Disruptions in splicing mechanisms can lead to diseases, including various cancers, highlighting its role in cancer progression.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • Gene splicing accurately identifies exonic sequences within large intronic regions.
  • Splicing relies on regulatory elements and trans-acting factors (RNA and proteins).
  • Errors in splicing can cause mRNA instability or aberrant protein products, linking splicing defects to diseases like cancer.

Purpose of the Study:

  • To review splicing regulatory mechanisms.
  • To explore how disruptions in these mechanisms contribute to cancer susceptibility and progression.

Main Methods:

  • Literature review of scientific articles on gene splicing and cancer.
  • Analysis of the interplay between splicing elements and trans-acting factors.
  • Examination of the consequences of splicing infidelity in disease.

Main Results:

  • Splicing fidelity is essential for cellular function and preventing disease.
  • Dysregulation of splicing pathways is implicated in the development and advancement of cancer.
  • Specific splicing alterations can promote tumor growth and metastasis.

Conclusions:

  • Understanding splicing regulation is key to comprehending cancer biology.
  • Targeting splicing mechanisms may offer novel therapeutic strategies for cancer treatment.
  • Further research into splicing's role in cancer is warranted.

More Related Videos

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Pre-mRNA Processing02:01

Pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
pre-mRNA Processing02:01

pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...