Related Experiment Video
Updated: Jul 10, 2026

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
Published on: March 28, 2018
A set of modular binary vectors for transformation of cereals
Axel Himmelbach1, Uwe Zierold, Götz Hensel
1Leibniz Institute of Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany.
Abstract:
Genetic transformation of crop plants offers the possibility of testing hypotheses about the function of individual genes as well as the exploitation of transgenes for targeted trait improvement. However, in most cereals, this option has long been compromised by tedious and low-efficiency transformation protocols, as well as by the lack of versatile vector systems. After having adopted and further improved the protocols for Agrobacterium-mediated stable transformation of barley (Hordeum vulgare) and wheat (Triticum aestivum), we now present a versatile set of binary vectors for transgene overexpression, as well as for gene silencing by double-stranded RNA interference. The vector set is offered with a series of functionally validated promoters and allows for rapid integration of the desired genes or gene fragments by GATEWAY-based recombination. Additional in-built flexibility lies in the choice of plant selectable markers, cassette orientation, and simple integration of further promoters to drive specific expression of genes of interest. Functionality of the cereal vector set has been demonstrated by transient as well as stable transformation experiments for transgene overexpression, as well as for targeted gene silencing in barley.
Related Concept Videos
Vectors in Engineering Applications
Vectors
Scalar and Vectors
Scalar quantities with the same physical units can be added or subtracted according to the usual algebra rules for numbers. For example, a class ending 10 min earlier than 50 min lasts...
Vector Representation of Complex Numbers
Consider a function defined as the product of the complex factors in the numerator divided by the product of the complex factors in the denominator.
Vector Algebra: Method of Components
In many applications, the magnitudes and directions of...
Vector Operations
A vector multiplied by a scalar value is called scalar multiplication. The result obtained is a new vector with a different magnitude. If the scalar is positive, the direction of the vector remains the same, but if it is negative, the direction of the vector is reversed. For example, the product of the mass and velocity yields the momentum.

