Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Tumor Immunotherapy01:27

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

Overview
Immune Response Against Viral Pathogens01:29

Immune Response Against Viral Pathogens

The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An Evolutionarily Conserved TUSC2-OSCP Interface Suggests a Mitochondrial Ca<sup>2</sup>⁺-Sensing Mechanism for ATP Synthase Regulation.

Journal of molecular biology·2026
Same author

Single-plate Runs of Mito Stress And Glycolysis Stress Tests using Adherent or Non-Adherent Cells.

Journal of visualized experiments : JoVE·2026
Same author

Adult-onset Still's disease diagnosed in pregnancy.

Modern rheumatology case reports·2026
Same author

Evaluation of GPT-5 in Periodontitis Staging and Grading: Retrospective Observational Study.

JMIR formative research·2026
Same author

Training biologists in Unix command-line skills: From curriculum to interactive online tutorials.

PLoS computational biology·2026
Same author

Effects of fibroblast growth factor 2 on muscle precursor cells from mouse limb and extraocular muscle.

PloS one·2026

Related Experiment Video

Updated: Jul 10, 2026

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
07:36

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice

Published on: June 12, 2021

CD8 T cell help for innate antitumor immunity.

Anil Shanker1, Grégory Verdeil, Michel Buferne

  • 1Centre d'Immunologie de Marseille-Luminy, Faculté des Sciences de Luminy, Aix-Marseille Université, Marseille, France.

Journal of Immunology (Baltimore, Md. : 1950)
|November 6, 2007
PubMed
Summary

Tumor-specific CD8 T cells help NK cells eliminate tumors, even those lacking the target antigen. This collaboration prevents the emergence of resistant tumor variants and metastasis.

More Related Videos

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
10:13

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells
09:57

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

Published on: January 29, 2019

Related Experiment Videos

Last Updated: Jul 10, 2026

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
07:36

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice

Published on: June 12, 2021

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
10:13

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells
09:57

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

Published on: January 29, 2019

Area of Science:

  • Immunology
  • Oncology
  • Cellular Biology

Background:

  • Innate immunity initiates adaptive antitumor responses.
  • The role of T cells in supporting NK cell-mediated tumor rejection is not fully understood.

Purpose of the Study:

  • To investigate the collaborative role of CD8 T cells and NK cells in tumor rejection.
  • To determine if T cell help is required for NK cell-mediated elimination of antigen-deficient tumor variants.

Main Methods:

  • Utilized P1A-specific CD8 T cells and P1A-expressing/deficient P815 mastocytoma cells in RAG-deficient mice.
  • Performed gene expression profiling of NK cells infiltrating tumors.
  • Assessed tumor rejection and emergence of antigen-negative variants.

Main Results:

  • P1A-specific CD8 T cells provide essential help to NK cells for rejecting P1A-deficient tumors.
  • Reconstitution of RAG-deficient mice with P1A-specific T cells conferred resistance to both tumor types when co-localized.
  • Elimination of antigen-negative variants required both activated T and NK cells.
  • NK cells infiltrating tumors showed an activated effector phenotype.

Conclusions:

  • Local tumor antigen-specific T cell-NK cell collaboration eliminates tumor cells regardless of antigen expression.
  • This collaboration contains the emergence of tumor escape variants before metastasis.