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Related Experiment Video

Updated: Jul 10, 2026

Osteoclast Derivation from Mouse Bone Marrow

Osteoclast Derivation from Mouse Bone Marrow

Published on: November 6, 2014

[Osteocalcin and bone].

Seiki Wada¹, Takeshi Fukawa, Sadahiro Kamiya

¹Josai International Universtiy, Faculty of Pharmaceutical Sciences, Department of Clinical Sciences.

Clinical Calcium

|November 6, 2007

Summary

Serum osteocalcin (OC) indicates bone formation rate. Assays measure intact and fragmented OC, but current measurements have limited value for primary osteoporosis evaluation.

Area of Science:

Biochemistry
Bone Metabolism
Endocrinology

Context:

Osteocalcin (OC) is a bone matrix protein produced by osteoblasts.
Serum OC levels reflect osteoblast activity and bone formation rates.
OC's interaction with hydroxyapatite involves gamma-carboxylation.

Purpose:

To develop an assay system for major circulating forms of osteocalcin (intact and N-terminal fragments).
To assess the clinical relevance of osteocalcin measurements in bone health.

Summary:

The assay measures intact and large N-terminal fragments of osteocalcin.
Undercarboxylated osteocalcin may indicate increased risk for osteoporotic fractures.
Current OC measurement does not replace bone mass assessment for primary osteoporosis.

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A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders

Published on: June 8, 2014

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Last Updated: Jul 10, 2026

Osteoclast Derivation from Mouse Bone Marrow

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A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders

Published on: June 8, 2014

Impact:

Provides a method for assessing key circulating osteocalcin forms.
Highlights the potential role of undercarboxylated OC in fracture risk.
Clarifies the current limitations of OC assays in managing primary osteoporosis.