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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: Jul 10, 2026

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia
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From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

Published on: October 19, 2014

Mapping lymphocyte plasma membrane proteins: a proteomic approach.

Matthew J Peirce1, Jeremy Saklatvala, Andrew P Cope

  • 1Kennedy Institute of Rheumatology Division, Imperial College London, UK.

Methods in Molecular Medicine
|November 7, 2007
PubMed
Summary

Identifying cell surface proteins is crucial for understanding rheumatoid arthritis (RA). This study presents a novel proteomic method to analyze plasma membrane proteins, aiding in the discovery of T-cell and macrophage interactions in RA.

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Single-Molecule Localization Microscopy of Membrane Proteins using Single-Antibody Labeling
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Single-Molecule Localization Microscopy of Membrane Proteins using Single-Antibody Labeling

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Last Updated: Jul 10, 2026

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia
10:18

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

Published on: October 19, 2014

Single-Molecule Localization Microscopy of Membrane Proteins using Single-Antibody Labeling
07:51

Single-Molecule Localization Microscopy of Membrane Proteins using Single-Antibody Labeling

Published on: March 20, 2026

Area of Science:

  • Immunology
  • Proteomics
  • Rheumatology

Background:

  • Tumor necrosis factor-alpha (TNF-alpha) plays a significant role in rheumatoid arthritis (RA) pathogenesis.
  • Direct cell contact between T-cells and macrophages in the RA joint may drive macrophage TNF-alpha production.
  • The specific ligand/receptor interactions mediating this cell contact are currently unknown.

Purpose of the Study:

  • To develop and validate a method for enriching and resolving plasma membrane (PM) proteins.
  • To identify PM proteins involved in T-cell and macrophage interactions relevant to RA.
  • To enable semiquantitative comparisons of PM proteins in resting versus activated cells.

Main Methods:

  • Cell surface biotinylation of murine T-cell hybridomas.
  • Differential centrifugation and streptavidin affinity capture for PM protein enrichment.
  • Solution phase isoelectric focusing followed by tandem mass spectrometry for protein identification.

Main Results:

  • Successfully identified 75 plasma membrane proteins.
  • Enabled semiquantitative comparison between resting and activated T-cells.
  • Demonstrated the method's applicability across various cell types.

Conclusions:

  • The developed proteomic method effectively enriches and resolves plasma membrane proteins.
  • This technique facilitates the identification of key proteins involved in cell-cell interactions in inflammatory diseases like RA.
  • The approach is versatile and applicable to diverse cell types for proteomic analysis.