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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: Jul 10, 2026

The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers

The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers

Published on: January 22, 2013

Forward-phase and reverse-phase protein microarray.

Yaping Zong¹, Shanshan Zhang, Huang-Tsu Chen

¹Full Moon BioSystems, Inc., Sunnyvale, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|November 7, 2007

Summary

Protein microarrays are vital for biomarker discovery and pathway analysis. New methods now preserve protein activity on slides, enabling reliable high-throughput proteomic research and accurate data analysis.

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Last Updated: Jul 10, 2026

The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers

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Published on: January 22, 2013

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Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

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Published on: October 23, 2019

Area of Science:

Proteomics
Biotechnology
Molecular Biology

Background:

Protein microarrays are essential tools for disease biomarker identification, therapeutic target discovery, and biological pathway analysis.
Maintaining the integrity and activity of proteins on microarray surfaces remains a significant challenge in proteomic research.
Advancements in research and development have led to methods for preserving the biological functions of spotted proteins.

Purpose of the Study:

To present general guidelines for preparing protein microarrays.
To enable efficient preparation of both forward- and reverse-phase protein microarrays.
To achieve reliable and accurate data in high-throughput proteomic analysis.

Main Methods:

Utilizing proprietary polymer-coated slides developed by Full Moon BioSystems (FMB).
Implementing general guidelines for protein sample preparation and spotting.
Employing unique detection and analysis technologies provided by FMB.

Main Results:

Successful preservation of biological functions for spotted proteins on slide surfaces.
Demonstrated ability to obtain reliable and accurate protein microarray data.
Facilitation of high-throughput analysis in proteomic studies.

Conclusions:

The developed guidelines and technologies enable efficient and effective protein microarray preparation.
Preservation of protein activity is achievable, leading to enhanced reliability in proteomic research.
Full Moon BioSystems offers a comprehensive solution for high-throughput proteomic analysis using protein microarrays.