Related Experiment Video
Updated: Jul 10, 2026

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
Published on: March 26, 2011
DNA extraction from fixed cytogenetic cell suspensions
M R Amorim1, F R Vargas, J C Llerena
1Divisão de Medicina Experimental, Instituto Nacional de Câncer, Rio de Janeiro, RJ, Brasil.
Abstract:
We developed a procedure for DNA extraction from small volumes of fixed cell suspensions previously prepared for conventional cytogenetic analysis. Good quality DNA was isolated with a fast and simple protocol using DNAzol reagent. This provided suitable DNA for various types of molecular analyses, including polymerase chain reaction, restriction fragment length polymorphism, denaturing high-performance liquid chromatography, and direct sequencing. This technique provides sufficient material for such test, which are important for diagnosis of neoplastic diseases in pediatric patients.
Related Concept Videos
DNA Isolation
DNA Isolation
DNA Agarose Gel Electrophoresis
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...

