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Published on: January 27, 2014
Screening for 2-quinolinone-derived selective androgen receptor agonists in doping control analysis.
Mario Thevis1, Maxie Kohler, Joachim Maurer
1Center for Preventive Doping Research-Institute of Biochemistry, German Sport University Cologne, Carl-Diem Weg 6, 50933 Cologne, Germany. m.thevis@biochem.dshs-koeln.de
Rapid Communications in Mass Spectrometry : RCM
|November 7, 2007
Summary
Selective androgen receptor modulators (SARMs) are banned doping agents. This study developed a mass spectrometry method for detecting SARMs and their metabolites in sports doping control, ensuring fair play.
Area of Science:
- Analytical Chemistry
- Pharmacology
- Sports Science
Background:
- Selective androgen receptor modulators (SARMs) are performance-enhancing drugs with misuse potential in sports.
- The World Anti-Doping Agency (WADA) bans SARMs as anabolic agents.
- Effective doping control requires sensitive and specific detection methods for SARMs and their metabolites.
Purpose of the Study:
- To synthesize and characterize model quinolinone-based SARMs.
- To investigate the mass spectrometric fragmentation pathways of these SARMs.
- To develop and validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) screening method for SARM detection in doping control.
Main Methods:
- Synthesis of four model 6-alkylamino-2-quinolinone SARMs.
- Electrospray ionization and collision-induced dissociation using an LTQ Orbitrap mass analyzer.
- Development of a screening procedure using triple quadrupole mass spectrometry (MRM, precursor ion scanning) after LLE sample preparation.
- LC-MS/MS analysis for detection limits, precision, and recovery.
Main Results:
- Established fragmentation pathways for quinolinone-based SARMs.
- Achieved low detection limits (0.01-0.2 ng/mL) for the developed LC-MS/MS method.
- Demonstrated good intra-day (3.2–8.5%) and inter-day (6.3–16.6%) precision.
- Obtained high recoveries (81–98%) with no significant ion suppression/enhancement effects.
Conclusions:
- The developed LC-MS/MS method is suitable for screening quinolinone-based SARMs and their metabolites in doping control.
- The method provides sensitive and reliable detection, aiding anti-doping efforts.
- Understanding fragmentation pathways is crucial for identifying novel SARMs and their metabolites.
