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Fibril-associated Collagen

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Related Experiment Video

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Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides
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Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides

Published on: January 31, 2014

D-periodic collagen-mimetic microfibers.

Shyam Rele1, Yuhua Song, Robert P Apkarian

  • 1Department of Surgery, Emory University School of Medicine, USA.

Journal of the American Chemical Society
|November 8, 2007
PubMed
Summary

Researchers developed a synthetic peptide that self-assembles into ordered, collagen-like fibrils. This breakthrough mimics native collagen structure, enabling precise engineering of periodic nano- and microscale features for advanced biomaterials.

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Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment
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Area of Science:

  • Biomaterials Science
  • Molecular Biology
  • Nanotechnology

Background:

  • Self-assembling peptides have formed various structures like membranes and filaments via electrostatic interactions.
  • Achieving highly ordered, collagen-like fibrils with D-periodic features from synthetic peptides remained a challenge.

Purpose of the Study:

  • To design and synthesize a novel peptide system capable of self-assembling into collagen-like fibrils with D-periodicity.
  • To explore the molecular mechanisms underlying peptide assembly and fibril formation.

Main Methods:

  • Design and synthesis of a specific oligopeptide sequence incorporating charged amino acids in a Xaa-Yaa-Gly motif.
  • Transmission electron microscopy (TEM) for visualizing the self-assembled fibrous structures.
  • Molecular dynamics (MD) simulations to investigate assembly mechanisms and stability.

Main Results:

  • A synthetic peptide system successfully self-assembled into a fibrous structure with well-defined D-periodicity, visualized by TEM.
  • Molecular dynamics simulations revealed that strong electrostatic and hydrogen bond interactions drive the formation of stable, triple-helical protomers and linear assemblies.
  • The peptide system effectively mimicked native collagen structure, producing D-periodic microfibers.

Conclusions:

  • This study presents the first synthetic peptide system to form highly ordered, D-periodic collagen-like fibrils.
  • The findings open new avenues for engineering linear assemblies with precise nano- and microscale periodic features.
  • This capability may facilitate the creation of advanced 2D and 3D fiber networks for various applications.