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Published on: March 24, 2015
Polymorphisms of interferon-gamma and interferon-gamma receptor 1 genes and pulmonary tuberculosis in Koreans
Jung Hye Hwang1, Eun Joo Kim, Su Yeon Kim
1Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Centre, Sungkyunkwan University School of Medicine, Seooul, Korea.
Background And Objective:
Many genetic variations are thought to be risk factors for the development of pulmonary tuberculosis (TB). The association of interferon-gamma (IFN-gamma) and IFN-gamma receptor 1 (IFN-gammaR1) gene polymorphisms with pulmonary TB is controversial. This study examined the association between IFN-gamma and IFN-gammaR1 gene polymorphisms and pulmonary TB among Koreans.
Methods:
Eighty patients with culture-confirmed pulmonary TB and 80 controls were studied. Polymorphisms of the IFN-gamma gene at position +874 were determined using the amplification refractory mutation system PCR assay, and the IFN-gammaR1 gene was genotyped at positions -611, -270, -56 and +95 employing matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using genomic DNA.
Results:
The genotype and allele frequencies of the IFN-gamma and IFN-gammaR1 gene polymorphisms did not differ significantly between the patients with pulmonary TB and controls.
Conclusions:
The IFN-gamma and IFN-gammaR1 gene polymorphisms do not appear to be responsible for host susceptibility to pulmonary TB in the Korean population.
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