Methods for the isolation and culture of human articular chondrocytes

The tissue engineering of cartilage implants may open new paths for the surgical treatment of joint surface defects. Autologous chondrocyte transplantation (ACT) has been gaining in clinical significance over the last several years. This study presents the methods used for isolation, monolayer culturing, multipication and assesment in transmission light microscopy of human chondrocytes. The tissue was gained from resected fragments of joint during total knee replacement.