Related Experiment Video
Updated: Jul 10, 2026
An Alternative to the Traditional Cold Pressor Test: The Cold Pressor Arm Wrap
Published on: January 16, 2014
The effect of chiropractic spinal manipulative therapy on salivary cortisol levels
Objective:
This study examines the effect of chiropractic spinal manipulative therapy on salivary cortisol levels.
Design:
Prospective case series over six weeks duration. The trial consisted of establishment of each individual's baseline cortisol level, a two week treatment period (4 treatments), and a two week post treatment period.
Setting:
Macquarie University Chiropractic Research Centre.
Participants:
Nine subjects (six male, three female), employed in a large corporation, volunteered to the trial of spinal manipulative therapy.
Main Outcome Measures:
Saliva samples were analysed using an Amerlex Radioimmunoassay Kit to determine the cortisol concentrations present.
Results:
Statistical interpretation, after exclusion of an apparent outlying subject, revealed results of statistical significance (p<0.001) for reduction of salivary cortisol over the complete five week study. In addition, there was no apparent alteration in salivary cortisol levels immediately preceding and 15 minutes after spinal manipulative therapy.
Conclusion:
The initial evidence is inconclusive, however, the potential relationship demands further investigation. Additional research is necessary in measuring the physiological effects of Chiropractic spinal manipulative therapy. This method is currently being used in a larger randomised controlled trial.
More Related Videos
08:25Collecting Saliva and Measuring Salivary Cortisol and Alpha-amylase in Frail Community Residing Older Adults via Family Caregivers
Published on: December 18, 2013
05:33Measuring Biophysical and Psychological Stress Levels Following Visitation to Three Locations with Differing Levels of Nature
Published on: June 19, 2019