Related Experiment Video
Updated: Jul 10, 2026
Synthesis of Strong Adhesive Hydrogel, Gelatin O-Nitrosobenzaldehyde
Published on: November 11, 2022
Bacterial adhesion to conventional hydrogel and new silicone-hydrogel contact lens materials
Laurent Kodjikian1, Emmanuelle Casoli-Bergeron, Florence Malet
1Department of Ophthalmology, Croix-Rousse University Hospital, 103, Grande rue de la Croix-Rousse, Lyon, France. kodjikian.laurent@wanadoo.fr
Background:
As bacterial adhesion to contact lenses may contribute to the pathogenesis of keratitis, the aim of our study was to investigate in vitro adhesion of clinically relevant bacteria to conventional hydrogel (standard HEMA) and silicone-hydrogel contact lenses using a bioluminescent ATP assay.
Methods:
Four types of unworn contact lenses (Etafilcon A, Galyfilcon A, Balafilcon A, Lotrafilcon B) were incubated with Staphylococcus epidermidis (two different strains) and Pseudomonas aeruginosa suspended in phosphate buffered saline (PBS). Lenses were placed with the posterior surface facing up and were incubated in the bacterial suspension for 4 hours at 37 degrees C. Bacterial binding was then measured and studied by bioluminescent ATP assay. Six replicate experiments were performed for each lens and strain.
Results:
Adhesion of all species of bacteria to standard HEMA contact lenses (Etafilcon A) was found to be significantly lower than that of three types of silicone-hydrogel contact lenses, whereas Lotrafilcon B material showed the highest level of bacterial binding. Differences between species in the overall level of adhesion to the different types of contact lenses were observed. Adhesion of P. aeruginosa was typically at least 20 times greater than that observed with both S. epidermidis strains.
Conclusions:
Conventional hydrogel contact lenses exhibit significantly lower bacterial adhesion in vitro than silicone-hydrogel ones. This could be due to the greater hydrophobicity but also to the higher oxygen transmissibility of silicone-hydrogel lenses.
