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Related Concept Videos

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: Jul 10, 2026

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders
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Conditional transgene expression mediated by the mouse beta-actin locus.

Ulrike Jägle1, Jürg A Gasser, Matthias Müller

  • 1Novartis Institutes for Biomedical Research, Novartis Pharma AG, Basel, Switzerland.

Genesis (New York, N.Y. : 2000)
|November 8, 2007
PubMed
Summary
This summary is machine-generated.

This study presents a novel method for precise gene insertion in mice using Cre/lox and Flp/FRT systems. This approach enables controlled transgene expression, advancing in vivo gene function studies.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Animal Models

Background:

  • Transgenic mice are crucial for studying gene function in vivo.
  • Position effects can hinder accurate tissue-specific transgene analysis.
  • A need exists for precise transgene integration and controlled expression.

Purpose of the Study:

  • To develop a versatile system for precise transgene integration into the mouse genome.
  • To enable conditional and locus-specific transgene expression.
  • To validate the system's efficacy for predictable gene expression.

Main Methods:

  • Combined Cre/lox and Flp/FRT recombination systems for transgene manipulation.
  • Utilized Flp/FRT-mediated cassette exchange in embryonic stem cells for transgene insertion.
  • Employed Cre-mediated excision of a floxed STOP cassette for conditional expression.
  • Validated the system using an EGFP reporter gene integrated into the beta-actin locus.

Main Results:

  • Successfully integrated EGFP reporter gene into the endogenous beta-actin locus.
  • Demonstrated Cre-mediated excision leading to conditional EGFP expression.
  • Observed predictable and strong EGFP expression profiles in various Cre mouse lines.
  • Validated the feasibility of spatially and temporally controlled transgene expression.

Conclusions:

  • The developed Cre/lox and Flp/FRT system allows for precise transgene integration and conditional expression.
  • This model facilitates predictable and robust transgene expression from the beta-actin locus.
  • The system enhances the study of gene function in vivo by overcoming position effects and enabling controlled expression.