Developmental patterns of macronutrient intake in female and male rats from weaning to maturity

Male and female Sprague-Dawley rats had available pure macronutrient diets, protein, carbohydrate and fat, from birth to day 77 of age. Analyses of their intake of these nutrients, as a function of age, demonstrate that, in both sexes, daily protein intake and preference for this nutrient relative to the other macronutrients rise steadily from weaning and peak precisely at the time of puberty (day 37-44 for females and days 42-49 for males), when there is also a peak in body weight gain. This is in contrast to daily carbohydrate intake, which peaks 2 weeks after puberty in males, and also to the female and male rats' preference for carbohydrate, which remains relatively stable from weaning to maturity. These patterns also differ from those observed for daily fat intake and fat preference for females and males, which are relatively high during the first postweaning week and then decline and remain very low until shortly after puberty (day 54), when there occurs a sharp burst in fat intake. Comparisons between the females and males reveal a significantly stronger preference for carbohydrate in the females, exhibited as early as 23 days of age; a stronger preference for protein and fat in the males, evident after day 28; and greater light-period feeding of carbohydrate and fat by females compared to males, apparent after puberty. Correlational analyses demonstrate that body weight and total kcal intake are closely related to daily protein consumption, more strongly in females compared to males; are strongly related to daily fat intake only in males; and are unrelated to intake of carbohydrate, at any age and in either sex.