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Updated: Jul 10, 2026
DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat
Published on: February 27, 2014
[Effect of schistosome ova on Trinitrobenzenesulfonic acid induced colitis in mice]
Jie Jiang1, Ru-yi Xue, Shun-cai Zhang
1Department of Gastroenterology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Objective:
To investigate the effects of intraperitoneal injected schistosome ova on TNBS-induced colitis and on the intestinal TLR4 expression in mice.
Methods:
40 BALB/c mice were randomized into 3 groups: normal control group (10 mice), TNBS group (20 mice) in which mice were exposed to trinitrobenzesulfonic acid (TNBS) and were induced with colitis, and the schistosome ova group (10 mice) in which mice were intraperitoneal injected with freeze-killed schistosome ova and later exposed to TNBS. The following variables were observed: mortality, pathological appearance of the colon, histological scoring of the specimen, serum TNF-alpha level, and intestinal TLR4 expression detected by RT-PCR and Immunohistochemistry.
Results:
Mortality of schistosome ova group was lower than that of the TNBS group (20% vs 70%, P < 0.05). Inflammation of the mice colon in the schistosome ova group was less severe than that of the TNBS group (1.4 +/- 0.5 vs 4.2 +/- 0.6, P < 0.01, Ameho criteria scoring). TLR4 expression of colon was up-regulated in mice of TNBS group and down-regulated in schistosome ova group which was still higher than that of normal controls (0.762 +/- 0.054 vs 0.325 +/- 0.029 vs 0.237 +/- 0.021, P < 0.01).
Conclusion:
Intraperitoneal injected schistosome ova can obviously reduce TNBS-induced colitis in mice, which may be attributed to down-regulated TLR4 expression in colon.
Insights
Intraperitoneal injection of schistosome ova reduced mortality and colon inflammation in mice with trinitrobenzesulfonic acid (TNBS)-induced colitis. This protective effect may be linked to the down-regulation of Toll-like receptor 4 (TLR4) expression.
Area of Science:
- Immunology
- Gastroenterology
- Parasitology
Context:
- Inflammatory bowel disease (IBD) models are crucial for understanding colitis pathogenesis.
- Trinitrobenzesulfonic acid (TNBS)-induced colitis is a widely used mouse model mimicking human Crohn's disease.
- Toll-like receptor 4 (TLR4) plays a significant role in inflammatory responses within the gut.
Purpose:
- To evaluate the therapeutic potential of schistosome ova in a TNBS-induced colitis model.
- To investigate the impact of schistosome ova on intestinal Toll-like receptor 4 (TLR4) expression during colitis.
Summary:
- BALB/c mice with TNBS-induced colitis were treated with intraperitoneal injections of freeze-killed schistosome ova.
- Schistosome ova treatment significantly reduced mortality rates (20% vs. 70%) and colon inflammation severity (1.4 vs. 4.2 score) compared to TNBS-only controls.
- TLR4 expression in the colon was downregulated in the schistosome ova group, suggesting a mechanism for reduced inflammation.
Impact:
- Schistosome ova demonstrate a protective effect against TNBS-induced colitis in mice.
- The findings suggest that modulating TLR4 expression via schistosome ova could be a novel therapeutic strategy for inflammatory bowel diseases.
- Further research into the immunomodulatory properties of schistosome ova could lead to new treatments for colitis.
