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Updated: Jul 10, 2026
Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
Published on: March 8, 2012
[Clone of HPV58 E6 gene and analysis of HLA-DQB1 03 restricted T-cell epitopes]
Ling Chen1, Zhu Shen, Jin-Jin Wu
1Department of Dermatology, Institute of Battle Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
Aim:
To clone HPV58 E6 gene and analyze the HLA-DQB1 03-restricted T cell epitopes on E6 protein.
Methods:
Total genome DNA was isolated from a cervical cancer sample. The HPV58 E6 gene was amplified by PCR and inserted into pGEM-T Easy vector. The recombinant plasmid was identified by restriction endonuclease analysis and sequencing. HLA-DQB1 03-restricted T cell epitopes on E6 protein were predicted and analyzed by the position-specific scoring matrix, support vector machine theory and prediction algorithm for proteasomal cleavages.
Results:
A HPV58 E6 gene was successfully cloned and submitted to GenBank (EF060239). Epitope 47(FADLRIAYRDGNPFA) and Epitope 102(RCIICQRPLCPQEKK) were theoretic HLA-DQB1 03-restricted T cell epitopes on E6 protein.
Conclusion:
These two epitopes could serve as candidates for screening and identification of the vaccine against HPV58 infection in the further study.
