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Related Experiment Video

Updated: Jul 10, 2026

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

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Published on: July 14, 2016

Haptocorrin in humans.

Anne L Morkbak¹, Steen S Poulsen, Ebba Nexo

¹Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark.

Clinical Chemistry and Laboratory Medicine

|November 10, 2007

Summary

Haptocorrin, a vitamin B12-binding protein, is found in many tissues, including fetal tissues. In vitamin B12 deficiency, analogues accumulate on haptocorrin, suggesting a role in monitoring B12 status.

Area of Science:

Biochemistry
Human Physiology
Evolutionary Biology

Background:

Haptocorrin (also known as R-binder) is the most recently evolved cobalamin-binding protein, originating from the intrinsic factor gene.
Its function and distribution in fetal and adult life are not fully understood, despite its known ability to bind cobalamin and analogues.

Purpose of the Study:

To investigate the tissue expression of haptocorrin in humans.
To explore the relationship between haptocorrin-bound analogues and vitamin B12 status.

Main Methods:

Immunohistochemistry was used to determine haptocorrin localization in fetal and adult tissues.
Enzyme-linked immunosorbent assay (ELISA) measured holo- and total haptocorrin in blood samples from individuals with varying vitamin B12 deficiency levels, confirmed by methylmalonic acid measurements.

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Main Results:

Haptocorrin was found in various exocrine glands, the gastrointestinal tract, and the respiratory system.
Individuals with vitamin B12 deficiency exhibited significantly higher levels of haptocorrin-bound analogues compared to non-deficient individuals.

Conclusions:

Haptocorrin is widely distributed, including in fetal tissues.
The accumulation of analogues on haptocorrin in vitamin B12 deficiency suggests its potential as a biomarker, warranting further investigation.