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Purification of a High Molecular Mass Protein in Streptococcus mutans
09:51

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Published on: September 14, 2019

Manganese affects Streptococcus mutans virulence gene expression.

P Arirachakaran1, E Benjavongkulchai, S Luengpailin

  • 1Chulalongkorn University, Bangkok, Thailand.

Caries Research
|November 10, 2007
PubMed
Summary

Manganese (Mn) influences the expression of Streptococcus mutans genes critical for virulence and biofilm formation. These effects vary depending on whether the bacteria are growing planktonically or in a biofilm.

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Area of Science:

  • Microbiology
  • Oral Health
  • Molecular Biology

Background:

  • Trace metal manganese (Mn) in drinking water and tooth enamel may promote dental caries.
  • Mn is essential for virulence factors in mutans streptococci, like the glucan-binding lectin (GBL) in Streptococcus sobrinus.
  • Streptococcus mutans glucan-binding proteins (Gbps), such as GbpC, are analogous to S. sobrinus GBL and contribute to biofilm structure and virulence.

Purpose of the Study:

  • To investigate the impact of manganese (Mn) on the transcription of Streptococcus mutans genes encoding glucan-binding proteins (Gbps), including gbpC.
  • To examine Mn's effects on other key Streptococcus mutans virulence genes.

Main Methods:

  • Microarray analysis to identify potential Mn effects on Gbp genes.
  • Northern and Western blot analyses, along with RT-PCR, to further investigate Mn's influence.
  • Experiments conducted on planktonic and biofilm cultures in Mn-depleted media and media with 50 µM Mn.

Main Results:

  • Manganese increased the expression of gbpC and gtfB while decreasing wapA in both planktonic and biofilm cultures.
  • Mn reduced gbpA and gbpD expression in biofilms only.
  • Mn enhanced gtfC expression in planktonic cultures but decreased spaP expression in biofilms.

Conclusions:

  • Manganese availability significantly impacts the expression of multiple Streptococcus mutans genes related to adhesion and biofilm development.
  • The observed effects of Mn on gene expression are dependent on the growth state (planktonic vs. biofilm) of Streptococcus mutans.