Related Experiment Videos
Visualization of proteases within a complex sample following their selective retention on immobilized bacitracin, a
J M van Noort1, P van den Berg, I E Mattern
1TNO-Medical Biological Laboratory, Rijswijk, The Netherlands.
Analytical Biochemistry
|November 1, 1991
Abstract:
A method for the visualization of individual proteases within a complex biological sample is described. In a single chromatographic step, proteases can be separated from other biomolecules by selective binding to immobilized bacitracin, a peptide antibiotic. Following desorption, proteases may be separated by SDS-polyacrylamide gel electrophoresis. The application of this method is presented in the visualization of proteases secreted by the fungus Aspergillus niger.